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BioWhittaker Molecular Applications neonatal normal human epidermal keratinocytes (nheks)
Binding of an anti-human Ep-CAM fluorescein-conjugated mAb to various cell lines analyzed by FACS. BKGEs (A2); human colorectal carcinoma cell lines COLO205 (A3), HCT116 (A4), and SW620 (A5); human prostate carcinoma cell lines PC3 (B1) and DU145 (B2); rat prostate tumorigenic cell line NRP154 (B3); HIAECs (B4); HUVECs (B5); lung HMVECs (C1); NHDFs (C2); human CASMCs (C3); neonatal <t>NHEKs</t> (C4); human malignant melanoma cell line A375 (C5); human breast carcinoma cell lines SkBr3 (D1), MCF7 (D2), and BT474 (D3); human rhabdomyosarcoma cell line A673 (D4); human liver carcinoma cell lines HepG2 (D5) and Hep3b (E1); T- and B-cell leukemia cell lines Jurkat (E2) and Bjab (E3), respectively; human lung carcinoma cell line SKMES1 (E4); and human neuroglioma cell line U87MG (E5) were incubated in the absence (thin line) or presence (thick line) of an anti-human Ep-CAM fluorescein-conjugated monoclonal antibody and analyzed by FACS.
Neonatal Normal Human Epidermal Keratinocytes (Nheks), supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of an anti-human Ep-CAM fluorescein-conjugated mAb to various cell lines analyzed by FACS. BKGEs (A2); human colorectal carcinoma cell lines COLO205 (A3), HCT116 (A4), and SW620 (A5); human prostate carcinoma cell lines PC3 (B1) and DU145 (B2); rat prostate tumorigenic cell line NRP154 (B3); HIAECs (B4); HUVECs (B5); lung HMVECs (C1); NHDFs (C2); human CASMCs (C3); neonatal NHEKs (C4); human malignant melanoma cell line A375 (C5); human breast carcinoma cell lines SkBr3 (D1), MCF7 (D2), and BT474 (D3); human rhabdomyosarcoma cell line A673 (D4); human liver carcinoma cell lines HepG2 (D5) and Hep3b (E1); T- and B-cell leukemia cell lines Jurkat (E2) and Bjab (E3), respectively; human lung carcinoma cell line SKMES1 (E4); and human neuroglioma cell line U87MG (E5) were incubated in the absence (thin line) or presence (thick line) of an anti-human Ep-CAM fluorescein-conjugated monoclonal antibody and analyzed by FACS.

Journal:

Article Title: Development of a Frozen Cell Array as a High-Throughput Approach for Cell-Based Analysis

doi:

Figure Lengend Snippet: Binding of an anti-human Ep-CAM fluorescein-conjugated mAb to various cell lines analyzed by FACS. BKGEs (A2); human colorectal carcinoma cell lines COLO205 (A3), HCT116 (A4), and SW620 (A5); human prostate carcinoma cell lines PC3 (B1) and DU145 (B2); rat prostate tumorigenic cell line NRP154 (B3); HIAECs (B4); HUVECs (B5); lung HMVECs (C1); NHDFs (C2); human CASMCs (C3); neonatal NHEKs (C4); human malignant melanoma cell line A375 (C5); human breast carcinoma cell lines SkBr3 (D1), MCF7 (D2), and BT474 (D3); human rhabdomyosarcoma cell line A673 (D4); human liver carcinoma cell lines HepG2 (D5) and Hep3b (E1); T- and B-cell leukemia cell lines Jurkat (E2) and Bjab (E3), respectively; human lung carcinoma cell line SKMES1 (E4); and human neuroglioma cell line U87MG (E5) were incubated in the absence (thin line) or presence (thick line) of an anti-human Ep-CAM fluorescein-conjugated monoclonal antibody and analyzed by FACS.

Article Snippet: Normal cells included: human iliac artery endothelial cells (HIAECs), human umbilical vein endothelial cells (HUVECs), human microvascular endothelial cells from lung (HMVECs), normal human dermal fibroblasts (NHDFs), human coronary artery smooth muscle cells (CASMCs), and neonatal normal human epidermal keratinocytes (NHEKs) purchased from BioWhittaker/Clonetics (San Diego, CA) and the bovine kidney glomerular endothelial cell (BKGEs) purchased from VEC Technologies, Inc. (Rensselaer, NY, USA).

Techniques: Binding Assay, Incubation